Agilent Infinity Series liquid chromatograph and 6420 triple quadrupole mass spectrometer

Here’s a picture of our LC-MS.

20150616_170729

Its a triple quadrupole instrument, so its designed for quantitative analysis of any compound you can solubilise and ionise, which includes pretty much anything relevant to metabolism, physiology, pharmacology and biochemistry.

Its a conventional pressure LC stack featuring thermostatted autosampler for 100 x 2ml vials, a thermostatted column compartment and a diode array detector at the bottom, if needed. We also have a fluorescence detector which isn’t shown.

We have two sources for the MS, a conventional electrospray ionisation [ESI] source and a Multi Mode Ionisation [MMI] source which can be set for full ESI, full Atmospheric Pressure Chemical Ionisation [APCI] or a mixture of the two, with full control over the corona current and charging voltage.

This instrument is currently analysing sugar derivatives and has previously been used to quantify phenolic compounds and anthocyanins in cherries, triglycerides in human plasma and serum and neonicotinoid pesticides. I am currently developing a method to target 15 bile acids in human samples and another to target lignin-derived phenolics in estuarine plant material and sediment.

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